Forwarded from Ron Paul
As 'Project Ukraine' Continues To Fail, A Desperate Europe Turns To Military Draft
Escalation in Ukraine continues, as a desperate Europe holding a losing hand turns toward drafting its youth to fight WWIII with Russia. Will sanity prevail? Also today, "Trump-proofing" military aid to Ukraine is hitting some European roadblocks.
Don't miss today's Liberty Report:
https://rumble.com/v4xzs7e-as-project-ukraine-continues-to-fail-a-desperate-europe-turns-to-military-d.html?mref=jcwo5&mc=2sdbb
Escalation in Ukraine continues, as a desperate Europe holding a losing hand turns toward drafting its youth to fight WWIII with Russia. Will sanity prevail? Also today, "Trump-proofing" military aid to Ukraine is hitting some European roadblocks.
Don't miss today's Liberty Report:
https://rumble.com/v4xzs7e-as-project-ukraine-continues-to-fail-a-desperate-europe-turns-to-military-d.html?mref=jcwo5&mc=2sdbb
π32β€4π«‘1
π¦ GAIN OF FUNCTION IS REAL:
HOW TO REVERSE ENGINEER THE SARSCoV2 VIRUS:
"A full-length infectious complementary DNA (cDNA) clone of a US SARS-CoV-2 clinical isolate WA1 was generated by cloning seven genomic fragments separately into vector plasmids (Figure 1 A). Additionally, two reporter viruses were constructed by replacing a 276-bp region in ORF7 with a green fluorescent protein (GFP) or a GFP-fused nanoluciferase (nLuc) gene (Figure 1A). After assembly into full-length cDNA, full-length RNA was electroporated into Vero-E6 cells (Scobey et al., 2013, Yount et al., 2003). After recovering the wild-type (WT), icSARS-CoV-2-GFP, and icSARS-CoV-2-nLuc-GFP recombinant viruses, viral replication was confirmed by the presence of sub-genomic-length leader-containing RNA transcripts 20 h after electroporation (Figure S1 ). All three recombinant viruses replicated (Figure S1), generated similar plaques in Vero E6 cells, and could be passaged serially in the cell culture without exogenous trypsin (Figure 1B). We defined cytopathic effect (CPE) by cell rounding and detachment from monolayers. GFP signals were evident in cells two days after transfection with RNA transcripts from both indicator viruses (Figure 1C).
"Our in vitro ligation method solves the stability issue by splitting unstable regions and cloning the fragmented genome into separate vectors, obviating the presence of a full-length genome (Yount et al., 2000). Our in vitro ligation strategy has generated reverse genetic systems for at least 13 human and animal coronaviruses and produced hundreds of mutant recombinant viruses (Beall et al., 2016, Menachery et al., 2015, Scobey et al., 2013, Xie et al., 2020, Yount et al., 2003). In contrast to other reports (Thao et al., 2020), reporter recombinant SARS-CoV-2 viruses generated herein replicated to normal WT amounts in continuous cell lines, allowing for robust ex vivo studies in primary cultures."
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250779/
ROBINMG π
HOW TO REVERSE ENGINEER THE SARSCoV2 VIRUS:
"A full-length infectious complementary DNA (cDNA) clone of a US SARS-CoV-2 clinical isolate WA1 was generated by cloning seven genomic fragments separately into vector plasmids (Figure 1 A). Additionally, two reporter viruses were constructed by replacing a 276-bp region in ORF7 with a green fluorescent protein (GFP) or a GFP-fused nanoluciferase (nLuc) gene (Figure 1A). After assembly into full-length cDNA, full-length RNA was electroporated into Vero-E6 cells (Scobey et al., 2013, Yount et al., 2003). After recovering the wild-type (WT), icSARS-CoV-2-GFP, and icSARS-CoV-2-nLuc-GFP recombinant viruses, viral replication was confirmed by the presence of sub-genomic-length leader-containing RNA transcripts 20 h after electroporation (Figure S1 ). All three recombinant viruses replicated (Figure S1), generated similar plaques in Vero E6 cells, and could be passaged serially in the cell culture without exogenous trypsin (Figure 1B). We defined cytopathic effect (CPE) by cell rounding and detachment from monolayers. GFP signals were evident in cells two days after transfection with RNA transcripts from both indicator viruses (Figure 1C).
"Our in vitro ligation method solves the stability issue by splitting unstable regions and cloning the fragmented genome into separate vectors, obviating the presence of a full-length genome (Yount et al., 2000). Our in vitro ligation strategy has generated reverse genetic systems for at least 13 human and animal coronaviruses and produced hundreds of mutant recombinant viruses (Beall et al., 2016, Menachery et al., 2015, Scobey et al., 2013, Xie et al., 2020, Yount et al., 2003). In contrast to other reports (Thao et al., 2020), reporter recombinant SARS-CoV-2 viruses generated herein replicated to normal WT amounts in continuous cell lines, allowing for robust ex vivo studies in primary cultures."
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250779/
ROBINMG π
PubMed Central (PMC)
SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract
The mode of acquisition and causes for the variable clinical spectrum of coronavirus disease 2019 (COVID-19) remain unknown. We utilized a reverse genetics system to generate a GFP reporter virus to explore severe acute respiratory syndrome coronavirus ...
π22π€¬7β€6π4π₯1
Forwarded from Going Underground
Genocidal neocon Nikki Haley visited Israel and wrote βfinish themβ on bombs destined to be dropped on Palestinians in Gaza, as Israel faces global outrage for its slaughter in Rafah
Haley shows once again that the slogan of the βrules-based orderβ is βKILL THEM ALLβ
Haley shows once again that the slogan of the βrules-based orderβ is βKILL THEM ALLβ
π€¬125π±8π5π€―4β€3
Media is too big
VIEW IN TELEGRAM
π18β€6π―2
Alastair Crooke: Are US and Russia Eyeball To Eyeball?
https://youtube.com/live/2MZTPw7I6n4
ROBINMG π
https://youtube.com/live/2MZTPw7I6n4
ROBINMG π
YouTube
Alastair Crooke: Are US and Russia Eyeball To Eyeball?
π15β€4π₯3
Forwarded from Quds News Network
This media is not supported in your browser
VIEW IN TELEGRAM
Thousands of demonstrators rally outside Downing Street in London in protest of the ongoing Israeli genocide in Gaza and the British government's complicity in the tragedy.
Video credit: @PSCUpdates
Video credit: @PSCUpdates
π95π20π7π₯4π€3π€¬1
This media is not supported in your browser
VIEW IN TELEGRAM
π₯70π15π―6π4π1π1
Forwarded from Vanessa Beeley
This media is not supported in your browser
VIEW IN TELEGRAM
Sunak taking complicity in Zionist genocide of Palestinians very seriously... Sunak should be in the Hague for enabling war crimes and supplying the weapons decapitating Palestinian children in Gaza.
π―107π14β€2π€―2π1
NOBEL PRIZE IN PHYSICS 2022 JOHN CLAUSER DEBUNKS THE "CLIMATE CRISIS"
Nobel Laureate John Clauser's Speech at the Competitive Enterprise Insti...
https://youtube.com/watch?v=fcnSXNb6qLw&feature=shared
ROBINMG π
Nobel Laureate John Clauser's Speech at the Competitive Enterprise Insti...
https://youtube.com/watch?v=fcnSXNb6qLw&feature=shared
ROBINMG π
YouTube
Nobel Laureate John Clauser's Speech at the Competitive Enterprise Institute
On March 18th, 2024, Dr. John Clauser - awarded the 2022 Nobel Prize in Physics - delivered a presentation at the Competitive Enterprise Institute in which he tackled the many false and misleading claims of climate activists and mainstream climatologists.
π36π―30π5β€4π1π1
Media is too big
VIEW IN TELEGRAM
China's Exposure of Silver Naked Short Selling, Could Bring Down Major US Banks - InfoWars
[Ignore the company advertisement at the end]
ROBINMG π
[Ignore the company advertisement at the end]
ROBINMG π
π31β€1
This media is not supported in your browser
VIEW IN TELEGRAM
π€ͺ27π17π11π€2π€―2β€1
Forwarded from @MTODAYNEWS π RT News new channel!!!
American fingerprints all over Rafah camp horror strike β CNN
Expert analysis of video footage suggests the deadly IDF airstrike on the Rafah tented refugee camp that killed at least 45 people was conducted with US weapons - despite President Biden insisting he wouldnβt allow their use in a Rafah offensive.
The tail of a Boeing-produced GBU-39 small-diameter bomb (SDB) - supposedly designed for high-precision attacks - is visible from footage reviewed by four explosive weapons experts for CNN.
π© @RTnews_unc3
Expert analysis of video footage suggests the deadly IDF airstrike on the Rafah tented refugee camp that killed at least 45 people was conducted with US weapons - despite President Biden insisting he wouldnβt allow their use in a Rafah offensive.
The tail of a Boeing-produced GBU-39 small-diameter bomb (SDB) - supposedly designed for high-precision attacks - is visible from footage reviewed by four explosive weapons experts for CNN.
Please open Telegram to view this post
VIEW IN TELEGRAM
π€¬73β€8π’4π3
Has anybody got a graph of Bitcoin valued in Silver over the last month to share so people can see what is really going on with Fiat alternatives?
ROBINMG π
ROBINMG π
π12π€ͺ1
Forwarded from MintPress News
This media is not supported in your browser
VIEW IN TELEGRAM
βThey did intend to at least seriously injure or kill her [Princess Diana]β¦she was about to go into campaigning on behalf of the Palestinians.β
Former MI5 officer Annie Machon on the alleged conspiracy to have the future Queen of England killed.
Former MI5 officer Annie Machon on the alleged conspiracy to have the future Queen of England killed.
π₯60π22π€10π€©1
Forwarded from Beboβs Friends (ΠΊΡΠΈΠΏ)
Media is too big
VIEW IN TELEGRAM
Tucker and Sachs!
Must watch
(58:34) What is a Neocon?
(1:25:28) Regime Change Never Works
(1:36:27) Who Blew up the Nord Stream Pipeline?
(2:01:45) COVID Origins
@MyLordBebo | Boost us! | X
Must watch
Jeffrey Sachs with probably the smartest and most accurate assessment of the Ukraine war, and American foreign policy more broadly, ever caught on tape.(20:17) Why did America push for Ukraine to Join NATO?
(58:34) What is a Neocon?
(1:25:28) Regime Change Never Works
(1:36:27) Who Blew up the Nord Stream Pipeline?
(2:01:45) COVID Origins
@MyLordBebo | Boost us! | X
β€31π13π₯4π1
Media is too big
VIEW IN TELEGRAM
π37π8π2
Forwarded from DD Geopolitics
This media is not supported in your browser
VIEW IN TELEGRAM
πΊπ¦π¬π§ Ukraine has already carried out strikes using British Storm Shadow missiles on Russian territory with London's approval, Yuri Sak, an advisor to the head of the Ministry of Strategic Industries, told Bloomberg.
"There has already been a precedent. Britain had previously allowed Ukraine to use their long-range Storm Shadow missiles, and we used them successfully," Sak said.
π΄ @DDGeopolitics
"There has already been a precedent. Britain had previously allowed Ukraine to use their long-range Storm Shadow missiles, and we used them successfully," Sak said.
Please open Telegram to view this post
VIEW IN TELEGRAM
π€¬65π6β€2π2π1